This study was conducted to determine the risk factors associated with nitrate/nitrite poisoning in cattle in the Burdur region. Feed and water samples were collected from 10 different farms randomly selected from 9 districts. A total of 33 feed samples, 13 water samples, 21 rumen contents, and 20 blood samples were collected for analysis. Nitrate and nitrite levels in feed, drinking water, and rumen fluid, as well as methemoglobin levels in blood samples, were determined using a spectrophotometric method. The average nitrate and nitrite levels in feed samples were 3.06 mg/kg and 3.54 mg/kg, respectively. The mean nitrate and nitrite concentrations in water samples were 1.04 mg/L and 0.06 mg/L, respectively, and 1.63 mg/L and 1.17 mg/L in rumen content. On the other hand, the average methemoglobin level in the blood samples was measured at 10.76%. The nitrite level in the feed used for cattle in the Burdur region was found to be considerably higher than the limits set by national feed regulations. While nitrate and nitrite concentrations in the water samples were within the limits recommended by the World Health Organization, the methemoglobin levels in the blood samples exceeded international standards. As a result, it was concluded that ration contents do not pose a risk of nitrate/nitrite poisoning in cattle in Burdur Province.